AChE (automatic reflex after AF-AFP)
ATTENTION: This test is being retired. Samples must be received by March 31, 2023, or your order will be canceled.


  • Open Spine/Abdominal Wall Defects

Clinical Utility

  • Fetal abnormalities suspected by ultrasound, such as spina bifida, ventral wall defects, anencephaly or abnormal maternal serum screening test result.
  • AF-AFP is performed on all amniocentesis samples drawn between 15-24 weeks gestation.
  • ACHE will automatically be done if the AF-AFP level is greater than or equal to 2.0 MoM.

Lab Method

  • Gel Electrophoresis

Test Code


CPT Codes*


ABN Required


Turnaround Time**

1-2 days

Preferred Specimen

10 mL Amniotic Fluid

*The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

**Reporting times are typical and begin once the sample(s) are received at the GeneDx laboratory, but could be extended in situations outside GeneDx’s reasonable control.